| Serie 1 |
| Martina Weil Restrepo | Universidad Católica | 7 | 56.52 | 1 q |
| Camila Letelier Ortega | Atlético San Vicente | 3 | 57.49 | 2 q |
| Danae Montserrat Ávila Retamal | Atlético Santiago | 5 | 59.93 | 3 q |
| Camila Ugarte Santander | Cultura Física - Viña del Mar | 2 | 1:01.02 | 4 q |
| Amanda Cerna Gamboa | Atlético Ñielol | 1 | 1:01.81 | 5 |
| Laura Belen Bustamante Gonzalez | Gerardo Manzanares | 4 | 1:03.13 | 6 |
| Maria Fernanda Salazar Romero | Oassi | 8 | 1:04.64 | 7 |
| Yasna Pacheco Diaz | Atlético Phoenix | 6 | 1:07.39 | 8 |
| Serie 2 |
| Bernardita Perez de Arce Lira | Universidad Católica | 4 | 59.21 | 1 q |
| Karina Alexandra Mendoza Vidal | Atlético Phoenix | 3 | 1:00.16 | 2 q |
| Pía Florencia Hachim Opazo | San Ambrosio | 5 | 1:01.12 | 3 q |
| Arami Cristina Fuenzalida Godoy | Universidad Austral | 6 | 1:01.67 | 4 q |
| Marjorie Beatriz Navarrete Del Valle | Atlético Universidad del Bio Bio | 2 | 1:06.18 | 5 |
| Fiona Amelie Hemke Hemke | Atlético Los Ángeles | 7 | 1:07.21 | 6 |
