| Serie 1 |
| Francisca Belen Rojas Hoffmann | Instituto Alonso de Ercilla | 4 | 11.43 | q |
| Belen Javer Suarez | Maitenes | 1 | 12.02 | q |
| Emilia Hernandez Acosta | Instituto Alonso de Ercilla | 3 | 12.70 | |
| Rosario Franzanni Marti | La Cruz de Machalí | 6 | 12.93 | |
| María Josefa Cardoen Larrain | Campanario - Buin | 2 | 13.02 | |
| Violeta Bersezio Cifuentes | La Misión de Calera de Tango | 5 | 13.98 | |
| Serie 2 |
| Martina Baeza Anguita | San Isidro | 1 | 12.25 | q |
| Guadalupe Rodriguez Leonvendagar | La Cruz de Machalí | 6 | 12.86 | |
| Paz Vial Perez | Villa María Academy | 2 | 13.24 | |
| Javiera Gonzalez Arellano | Institución Teresiana | 4 | 13.27 | |
| Sofias Torres Maggi | Highlands | 5 | 13.63 | |
| Sofía Ignacia Romero Abbott | Campanario - Buin | 3 | 14.11 | |
| Serie 3 |
| Matilde Paz Poblete Hormazabal | Campanario - Buin | 1 | 12.47 | q |
| Colomba Montero Lopez | Villa María Academy | 3 | 12.60 | |
| Constanza Achondo Roman | Villa María Academy | 2 | 12.93 | |
| Julieta Alegria O'ryan | San Francisco de Paine | 6 | 13.61 | |
| Catalina Fernanda Duque González | San Francisco de Paine | 5 | 13.90 | |
| Laura Sofía Menendez Ruiz | San Francisco de Paine | 4 | DNF | |
| Serie 4 |
| Isidora Ibarra Cortìnez | San Isidro | 6 | 12.29 | q |
| Amparo Zamora Cornejo | Maitenes | 1 | 12.75 | |
| Fernanda Ortuzar Canobra | San Francisco de Paine | 5 | 12.96 | |
| Amalia Rosenfeld Pavicevic | Maitenes | 4 | 13.11 | |
| Antonia Clericus Estibill | Campanario - Buin | 2 | 13.19 | |
| Maria Ignacia Carrera Fumeron | Instituto Alonso de Ercilla | 3 | | |
| Serie 5 |
| Montserrat Vega García | La Cruz de Machalí | 2 | 12.40 | q |
| María Hevia Tagle | Villa María Academy | 4 | 13.03 | |
| Teresita Espinola Lazo | Villa María Academy | 1 | 13.87 | |
| Isidora Diaz Barranco | Highlands | 3 | 13.93 | |
| Valentina Ruiz Sanchez | Institución Teresiana | 5 | | |
| Emilia Naser Barros | Highlands | 6 | | |
| Serie 6 |
| Olivia Lyon Gaete | San Isidro | 2 | 11.95 | q |
| María Del Rosario Ariztía Lecaros | San Isidro | 6 | 13.20 | |
| Maria Gracia Sepulveda Mena | Highlands | 4 | 14.08 | |
| María De Los Ángeles Fernandez Valdes | Highlands | 1 | 14.78 | |
| Daniela Abigail Madrid Toledo | Institución Teresiana | 3 | | |
| Bernardita Pérez Lyon | San Isidro | 5 | | |
| Serie 7 |
| Antonia Gabriela Padilla Peña | Instituto Alonso de Ercilla | 2 | 11.88 | q |
| María Jesus Gana Hollemart | Maitenes | 3 | 12.49 | |
| Emilia Sáez Rodríguez | La Cruz de Machalí | 4 | 12.97 | |
| Norma Morales Ratto | La Misión de Calera de Tango | 6 | 13.14 | |
| Clarita Canales Gana | Maitenes | 5 | 14.90 | |
